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U.S. Measurement System

Improved Antibody-Based Metrology in Flow Cytometry
February 23, 2006
Building 101, Lecture Room A, NIST

This U.S. Measurement System (USMS) workshop is one of a series to assess the nation's needs in the area of standards and technologies for immunoassay-based measurements. Flow cytometry (FC) is an important tool in clinical medicine and cancer and immunology research. The objective of this USMS workshop is to assess barriers to technological innovation in quantitative cytometer measurements in the U.S. At present, cytometers are used in clinical laboratories to estimate the relative number of specific receptors expressed on the surface of lymphocytes from the blood, lymphonodes, bone marrow, and other body fluids. The number and type of receptors are indicators of the lineage, clonality, stage of differentiation, and state of activation. Technological advances are needed to realize quantitative FC measurements to provide more accurate measurements of the absolute number of specific receptors expressed on the surface of cells. Quantitative measurements are also critical for multiple fluorescence channel, multiplexed bead, and imaging cytometers. The USMS workshop aims to bring together leaders in cytometry-based medical diagnostics, diagnostic testing, clinical laboratory medicine, and regulatory affairs to identify the fundamental and common metrology barriers to advances in flow cytometry. In addition, the workshop(s) will aim to foster partnerships and collaborations for development of the infrastructural metrology needed to insure scientifically sound measurements.

Agenda:

8:30 a.m. -- Welcome and Introductory remarks: Whetstone, NIST
8:40 a.m. -- Goal of the workshop: M. Amos, A Gaigalas, L. Wang, NIST

9:00-10:20 a.m. -- Instrument performance: Lili Wang, NIST

9:20 a.m. -- Bob Hoffman, BD
9: 40 a.m. -- Eric Chase, Cytek
10.00 a.m. -- Marc Cheetham, Coulter
10:20 a.m. -- James Jacobson, Luminex

10:20-10:30 a.m. -- Break -10 minutes

10:30-11:40 a.m. -- Bead manufactures: Moderator - R. Zucker, EPA

10:30 a.m. -- Brinkley, Duke
10:40 a.m. -- Chad Owens, Bangs-Polyscience
10:50 a.m. -- Bill Godfrey, Molecular Probes
11: 00 a.m. -- Jeff Wang, Spherotech
11: 10 a.m. -- Coulter beads-

11:20 a.m. -- BD beads: Bert Houltz

11:40 a.m.-12:30 p.m. -- Working Lunch

12:30-2:15 p.m. -- Users: Moderator - Gerald Marti, FDA

12:30 p.m. -- Janet Ottinger, Duke
12 :45 p.m. -- Robert Zucker, EPA
1:00 p.m. -- Steve Prefetto, NIH
1:15 p.m. -- Maurice O'Gorman, Northwest U
1:30 p.m. -- Norman Purvis, Cytometry Associates Nashville Tennessee
1:45 p.m. -- Albert Donnerberg, University of Pittsburgh
2:00 p.m. -- Darin Hickerson, Aldagen Durham NC
2:15 p.m. -- Bob Vogt, CDC

Moderated discussion 2:15 p.m. - 5:00 p.m.

2:15 p.m. -- Moderator - Robert Zucker

The purpose of the discussion is to identify a few specific tasks for improving the reliability of cytometer measurements on a national scale. The tasks would be accomplished by cooperative work involving academic, industrial and government laboratories. Formation of working group for Flow Cytometry QA and standards. Issues in education.

5:00 p.m. -- Adjournment

Contacts:

Dr. Adolfas Gaigalas, NIST
(301) 975 2873, adolfas.gaigalas@nist.gov

Dr. Lili Wang, NIST
(301) 975 2447, lili.wang@nist.gov

Dr. Michael Amos, NIST
(301) 975 8631, michael.amos@nist.gov

Dr. Robert M. Zucker, EPA
(919) 541 1585, zucker.robert@epa.gov

Dr. Gerald Marti, FDA
(301) 827 0453, gemarti@helix.nih.gov

Note: Teleconference facilities will be available in Lecture Room A.

 

General inquiries on the USMS Workshop program may be forwarded to usms_workshops@nist.gov.

Last updated: July 9, 2007