PRINTABLE BROCHURE
AVAILABLE AT:
http://usms.nist.gov/workshops/proteomics_brochure.pdf
MEASUREMENT CHALLENGES IN PROTEOMICS
Roadmapping America's Measurement Needs in the Protein Sciences
March 12,
2006
Sheraton Boston Hotel
Boston, Massachusetts
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Organizing
Committee
Executive Committee David
Bunk, PhD Stanley
Hefta, PhD William
F. Koch, PhD John
Kozarich, PhD Joshua
LaBaer, MD, PhD James
R. (Rick) Ludwig, PhD Gilbert
S. Omenn, MD, PhD Henry
Rodriguez, PhD, MBA Sudhir
Srivastava, PhD Thomas
Wiggins BSME, MBA Advisory Board Gradimir
Georgevich, PhD Doug
Jones, MS, MBA Stephen
Stein, PhD Illarion
V. Turko, PhD
Kathleen
Kilmer |
Charge
to Participants
The word "proteome" is derived from PROTEins expressed by a genOME, and it refers to all the proteins produced by an organism, much like the genome is the entire set of genes. The human body contains a vast number of different proteins, each having different functions. As the main components of the physiological pathways of the cells, proteins serve vital functions in the body. Proteomics plays an important role in drug discovery, diagnostics and molecular medicine, providing a link between genes, proteins, metabolites, and disease. The National Institute of Standards and Technology (NIST) is facilitating a U.S. MEASUREMENT SYSTEM (USMS) INITIATIVE that will be undertaken in close cooperation with the private and public sectors to ensure that the nation's highest-priority measurement needs are identified and met in the 21st century. NIST plans to publish a USMS Roadmap on a regular basis, reporting to USMS customers and stakeholders on what should be done, by NIST and others, to meet the needs of the USMS, and delineating the consequences of not meeting those needs. The Measurement Challenges in Proteomics Workshop is part of this initiative and will endeavor to survey (identify and prioritize) system-wide needs in coordinating, performing and using protein measurements. The inability to establish performance criteria to better understand the quality of proteomic technique results, has led to poor confidence in protein measurement techniques, difficulty in assessing the agreement of different experiments, conflicting reports in the literature, and lost opportunities. If proteomics technologies are to successfully make their way into clinical diagnostics, universally accepted metrics will be needed at many steps along the way to help clarify experimental results and protocols and make them comparable. By participating
in this workshop, members will help identify and prioritize a measurement
needs agenda that addresses the technical infrastructure (measurement
science, standards, and data) in the field of proteomics. Participants
will also identify how best to transfer knowledge and share priorities
across industry, clinical laboratories, government, funding agencies,
regulatory agencies, educational and not-for-profit institutions in order
to build strong collaborations and partnerships. By the end of the workshop,
a roadmap of the measurement needs should be outlined. The steps needed
to address these needs as well as the consequences of inaction will be
stated. |
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National
Institute of Standards and Technology
U.S. Department of Commerce U.S. Human Proteome Organization Measurement Challenges in Proteomics Workshop March 12, 2006 Boston
Sheraton Hotel AGENDA |
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7:00 AM – 7:45 AM Registration and Continental Breakfast
7:50 AM – 8:00 AM Welcome and Introduction
David Bunk, PhD
National Institute of Standards and Technology
Henry Rodriguez, PhD, MBA
National Cancer Institute
8:00 AM – 8:10 AM NIST and the Science of Measurement
Willie E. May, PhD
National Institute of Standards and Technology
8:10 AM – 8:20 AM US Measurement System Initiative
Thomas Wiggins BSME, MBA
National Institute of Standards and Technology
8:20 AM – 8:40 AM A Need for Measurement Standards in Proteomics
(HUPO PPP and NCI EDRN)
Gilbert S. Omenn, MD, PhD
University of Michigan
Sudhir Srivastava, PhD
National Cancer Institute
8:40 AM – 9:00 AM Steps & Complexities Involved In Measuring Proteins/Peptides
Daniel W. Chan, PhD
Johns Hopkins Medical Institutions
9:15 AM - 3:30 PM BREAKOUT SESSIONS
SESSION 1
Sample Preparation and Protein Separation
Successful protein separation is mainly dependent upon decisions made regarding sample preparation. Identifying the pros and cons of common and emerging methodologies and technologies used in sample preparation (collection, processing and storage) and protein separation is the first step in achieving results that are accurate and reproducible.
Lead Moderator: Andrew Link, PhD
Vanderbilt Medical Center
Co-Moderators: Gilbert S. Omenn, MD, PhD
University of Michigan
Thomas Wiggins BSME, MBA
National Institute of Standards and Technology
Facilitator: Herbert F. Barber
National Institute of Standards and Technology
SESSION 2
Protein Identification and Quantification
A high degree of confidence in the identification and quantification of proteins and their posttranslational modifications - in complete expression profiles or in the context of defined protein complexes – remains a challenge to proteomics research and a concern to regulatory agencies and the industrial community. A key requirement for this approach is the ability to resolve the individual components of complex protein and peptide mixtures with a high level of confidence across multiple platforms. Criteria to be assessed include sensitivity and selectivity, reproducibility and robustness, and suitability for compliance with current guidelines. This session will address the technical issues encountered in protein identification and quantification.
Lead Moderator: David R. Goodlett, PhD
University of Washington
Co-Moderators: Stanley Hefta, PhD
Bristol-Myers Squibb
David Bunk, PhD
National Institute of Standards and Technology
Facilitator: Mike Martin
National Institute of Standards and Technology
SESSION 3
Proteomic Assay Development and Validation (multiplex/functional/High throughput)
Proteomic biomarkers need to be validated (analytically and biologically) and developed into robust assays. Given the chemical and structural complexity of the proteome, the development of proteomic high throughput assays has been challenging. Validation of an analytical method identifies the sources of potential error and quantifies the performance characteristics of an assay. Validation of the technology/method will ensure that it provides reliable information for the intended diagnostic application, and therefore is a key component of the development and approval process. Addressing the issues encountered in multiplex and functional proteomic assay development and validation will help provide guidance for the selection of analytical techniques and their optimal use.
Lead Moderator: Joshua LaBaer, MD, PhD
Harvard Institute of Proteomics
Co-Moderators: John Kozarich, PhD
ActivX Biosciences
Henry Rodriguez, PhD, MBA
National Institute of Standards and Technology
Facilitator: Rick Ludwig, PhD
INCAPS
SESSION 4
Study Design and Experimental Design
Proper study design and experimental design is a requisite for clinical proteomics. However, a lack of uniformity and standards in data collection, data interpretation, and inadequate sample size with associated demographic and clinical information has led to irreproducibility of data, uncertainties, and bias-rich results. This session will address the variables that affect confounding and bias results and ways to minimize them.
Lead Moderator: Jane C. Schroeder, DVM, PhD
University of North Carolina School of Public
Health
Co-Moderators: Sudhir Srivastava, PhD
National Cancer Institute
Paul Wagner, PhD
National Cancer Institute
Facilitator: Willie May, PhD
National Institute of Standards and Technology
For each session:
9:15 AM - 10:20 AM Identifying the Issues/Needs in Proteomics
10:20 AM - 10:40 AM Coffee Break
10:45 AM – 11:50 AM Clarifying, Refining and Combining the Issues/Needs
Noon – 1:15 PM Lunch
1:20 PM – 3:30 PM - Prioritize the Issues/Needs in Order of Importance
- Recommend Approaches to Solve
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3:30 PM – 3:50 PM Soda Break
4:00 PM – 6:00 PM Outcomes/Recommendations presented to workshop attendees
6:00 PM Adjourn
| March
12, 2006
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http://usms.nist.gov/workshops/proteomic.htm ------------------------ Breakout Session
Selection Registration Fee: $125 Hotel Information Nestled in charming and historic Back Bay, the Sheraton Boston Hotel is conveniently located from Boston's Logan Airport, connected to the Hynes Convention Center and to 200 shops at the Prudential Center and Copley Place Mall. Registrants are
encouraged to reserve their rooms by contacting the hotel directly. When
booking your stay make sure to mention that you will be attending the
Measurement Challenges in Proteomics workshop. A number of rooms have
been reserved at a rate of $189/night + tax. |